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If gas is NOT present, then add 3 drops of sufanilic acid (reagent A) and 3 drops of naphthlamine (reagent B) to your tube. Mix gently by tapping the bottom of the tube and wait 2-3 minutes. If your tube turns red, then bacteria is positive for nitrate reductase and the following reaction has occurred: 2.Answers is the place to go to get the answers you need and to ask the questions you wanto Note: Let tube clot for 20-30 minutes before centrifugation • Red Top Tube with Yellow Ring: o Contains a clot activator with a gel separator o Also called "Red Gel" or "Serum Gel" in Test Catalog o Used for the collection of various laboratory tests and special testing o Do NOT freeze serum on separator gel.RedTube is a pornographic video sharing site, which in September 2009 held an Alexa ranking within the world's top 100 sites. [3] It is one of several pornographic websites owned by Mindgeek. In June 2010 it had fallen out of the top 100, but it made a return in mid-2012. 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